An annular-flow, hollow-fiber membrane chromatography device for fast, high-resolution protein separation at low pressure

High-resolution protein separation is currently carried out using columns packed with ultrafine particulate chromatographic media which can only be operated at very high pressures. This restricts the flow rate and thereby the speed of separation. A membrane with ultrafine pores shares the same mass transport advantages as a bed of ultrafine particles. However, application of membrane chromatography has largely been restricted to low-resolution …