Rapid Embedding in Lowicryl K4M for Immunoelectron Microscopic Studies

Abstract A rapid method of embedding tissues in Lowicryl K4M was developed. The initiator was replaced with benzoin ethyl ether, and the temperature of polymerization under indirect ultraviolet light was increased from −35°C to −10°C. The complete polymerization occurred in 24 hours. Compared with the original method and two other rapid methods proposed by Altman et al. (J. Histochem. Cytochem. 32:1217–1223, 1984), the above mentioned rapid …