Journal article
Enzymatic Litmus Test for Selective Colorimetric Detection of C–C Single Nucleotide Polymorphisms
Abstract
A paper based litmus test has been developed using modulation of urease enzyme activity for detection of C-C mismatch single nucleotide polymorphisms (SNPs) by the naked eye. Urease is first inactivated with silver ions and printed onto paper microzones. Addition of DNA containing C-C mismatches reactivates urease via binding of Ag(I), allowing restoration of urease activity, hydrolysis of urea to produce ammonia, and an increase in pH, which …
Authors
Wolfe MG; Ali MM; Brennan JD
Journal
Analytical Chemistry, Vol. 91, No. 7, pp. 4735–4740
Publisher
American Chemical Society (ACS)
Publication Date
April 2, 2019
DOI
10.1021/acs.analchem.9b00235
ISSN
0003-2700