Electron spectroscopic imaging of encapsidated DNA in vaccinia virus Journal Articles

abstract

• We have used electron spectroscopic imaging to locate the phosphorus in vaccinia DNA in situ in unstained, ultrathin sections of virions. The phosphorus of the DNA backbone appeared to form a halo on the core periphery surrounding a phosphorus-impoverished central element. These results constrain models for how DNA could be packaged into mature vaccinia particles.Key words: vaccinia, electron spectroscopic imaging, DNA.

authors

• Harauz, G
• Evans, DH
• Beniac, DR
• Arsenault, Larry
• Rutherford, B
• Ottensmeyer, FP

status

• published 

publication date

• October 1, 1995

has subject area

• 0605 Microbiology (FoR)
• 0707 Veterinary Sciences (FoR)
• 1108 Medical Microbiology (FoR)
• DNA, Viral (MeSH)
• Electron Probe Microanalysis (MeSH)
• Microbiology (Science Metrix)
• Microscopy, Electron (MeSH)
• Phosphorus (MeSH)
• Vaccinia virus (MeSH)
• Virion (MeSH)

published in

• Canadian Journal of Microbiology  Journal

keywords

• DNA, Viral
• Electron Probe Microanalysis
• Microscopy, Electron
• Phosphorus
• Vaccinia virus
• Virion

Digital Object Identifier (DOI)

• 10.1139/m95-122

PubMed ID

• 8590404

start page

• 889

end page

• 894

volume

• 41

issue

• 10