Hemoglobin Hamilton or α2β211(a8)Val→Ile: A silent β‐chain variant detected by triton X‐100 acid‐urea polyacrylamide gel electrophoresis Journal Articles uri icon

  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All


  • AbstractA silent β‐chain hemoglobin variant, not detectable by starch gel or agar gel electrophoresis was found using Triton X‐100 acid‐urea polyacrylamide gel electrophoresis. The abnormal β‐X chain had a more anodic electrophoretic mobility; 38% of the total β chain was of the abnormal type. Structural analysis using high‐performance liquid chromatography and microsequencing procedures indicated a valine to isoleucine substitution at position β11(A8). This anomaly did not change the functional proper ties of the hemoglobin molecule. A mild reticulocytosis was observed in the propositus.


  • Wong, SC
  • Ali, Mahmoud
  • Lam, H
  • Webber, BB
  • Wilson, JB
  • Huisman, THJ

publication date

  • January 1984