Hemoglobin Hamilton or α2β211(a8)Val→Ile: A silent β-chain variant detected by triton X-100 acid-urea polyacrylamide gel electrophoresis Academic Article uri icon

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abstract

  • A silent beta-chain hemoglobin variant, not detectable by starch gel or agar gel electrophoresis was found using Triton X-100 acid-urea polyacrylamide gel electrophoresis. The abnormal beta-X chain had a more anodic electrophoretic mobility; 38% of the total beta chain was of the abnormal type. Structural analysis using high-performance liquid chromatography and microsequencing procedures indicated a valine to isoleucine substitution at position beta 11(A8). This anomaly did not change the functional properties of the hemoglobin molecule. A mild reticulocytosis was observed in the propositus.

authors

  • Wong, SC
  • Ali, Mahmoud
  • Lam, H
  • Webber, BB
  • Wilson, JB
  • Huisman, THJ

publication date

  • January 1984