Journal article
Role of calcium in stabilizing the structure of hyalin, a major protein component of the sea urchin extraembryonic hyaline layer
Abstract
The interactions of NaCl and CaCl2 with the sea urchin embryo coat protein hyalin were investigated. Endogenous protein tryptophan fluorescence was enhanced by almost 45% in the presence of 200mM NaCl while 1mM CaCl2 reversed this effect and brought the intensity of fluorescence back close to that of the native protein. Half-maximal concentrations of 53 and 0.32mM were determined for NaCl and Ca+2, respectively. Hyalin conformation, as measured …
Authors
Robinson JJ; Taylor L; Ananthanarayanan VS
Journal
Biochemical and Biophysical Research Communications, Vol. 152, No. 2, pp. 830–836
Publisher
Elsevier
Publication Date
April 1988
DOI
10.1016/s0006-291x(88)80113-x
ISSN
0006-291X