Purification of vesicular stomatitis virus and the analysis of P32-labeled viral components
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Vesicular stomatitis virus, grown in vitro in Earle’s L cells, was purified using a caesium chloride equilibrium gradient. The infectious virus particles were found in a narrow zone about a density of 1.20 g/ml. The use of isotopically labelled virus, first partially purified by differential centrifugation, showed that a second, less dense, but noninfectious band of radioactive material was also present. A chemical analysis of the purified infectious material showed that VSV is an RNA virus containing a high proportion of phospholipid. These results were confirmed by IUDR and chloroform sensitivity studies. The material in the less dense, noninfectious band had a chemical constitution similar to that of the infectious particles.
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