[2] Techniques for human adenovirus vector construction and characterization

This chapter describes the techniques for human adenovirus vector construction and characterization. Human adenoviruses (Ads) have attracted considerable attention lately for their potential use as vectors for gene therapy, for recombinant vaccines, and for high-level protein expression in mammalian cells. Among the reasons for these interests are the following: (1) the 36 kilobase pair (kbp) double-stranded DNA genome of Ad is relatively easy …