Journal article
Hypersensitive PCR, Ancient Human mtDNA, and Contamination
Abstract
When highly efficient polymerase was used with high cycle numbers (50-60), strong amplifications were observed, but negative controls were also unexpectedly amplified in a study of ancient human mtDNA from 2000-year-old skeletons. The results of a series of tests revealed that the hypersensitive polymerase chain reaction (PCR) generated by higher cycles and the presence of contaminant DNA (though at extremely low levels) should be responsible …
Authors
Yang DY; Eng B; Saunders SR
Journal
Human Biology, Vol. 75, No. 3, pp. 355–364
Publisher
Johns Hopkins University Press
Publication Date
2003
DOI
10.1353/hub.2003.0050
ISSN
0018-7143