Antifreeze proteins from the ocean pout, Macrozoarces americanus: circular dichroism spectral studies on the native and denatured states

CD spectral data on the antifreeze proteins from the ocean pout (Macrozoarces americanus) have been obtained under native and denaturing conditions. At low temperatures (near 0°C), the far-ultraviolet CD spectra of the two known immunologically distinct groups of ocean pout proteins are found to be qualitatively similar to those of the antifreeze glycoproteins, with a distinct positive band near 225 nm. However, unlike the latter, the ocean …