Regulated Expression of a Mammalian Nonsense Suppressor tRNA Gene In Vivo and In Vitro Using the lac Operator/Repressor System

We have exploited the Escherichia coli lac operator/repressor system as a means to regulate the expression of a mammalian tRNA gene in vivo and in vitro. An oligonucleotide containing a lac operator (lacO) site was cloned immediately upstream of a human serine amber suppressor (Su+) tRNA gene. Insertion of a single lac repressor binding site at position −1 or −32 relative to the coding region had no effect on the amount of functional tRNA made …