Fluorogenic DNAzyme Probes as Bacterial Indicators

Abstract

Lighting up bacteria: An RNA‐cleaving fluorescent DNAzyme (RFD) can produce a fluorescent signal in the crude extracellular mixture generated by live bacterial cells (see picture). These DNAzymes cleave a lone RNA linkage (R) embedded in a DNA chain and flanked by nucleotides labeled with a fluorophore (F) and a quencher (Q).

Authors

Ali MM; Aguirre SD; Lazim H; Li Y

Journal

Angewandte Chemie International Edition, Vol. 50, No. 16, pp. 3751–3754

Publisher

Wiley

Publication Date

April 11, 2011

DOI

10.1002/anie.201100477

ISSN

1433-7851

Associated Experts

Yingfu Li

Professor, Faculty of Health Sciences

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