Journal article
The modus operandi of a DNA enzyme: enhancement of substrate basicity
Abstract
BACKGROUND: We wished to investigate the mode of catalytic action of a small DNA enzyme (DNAzyme), PS5.M, that, when folded into an appropriate tertiary structure, catalyzes the insertion of copper and zinc ions into porphyrins such as mesoporphyrin IX (MPIX). PS5.M, originally derived from SELEX experiments for specific DNA binders of the distorted porphyrin N-methylmesoporphyrin (NMM), had enzymatic parameters (kcat/K(m) = 39,700 M-1 min-1; …
Authors
Li Y; Sen D
Journal
Cell Chemical Biology, Vol. 5, No. 1, pp. 1–12
Publisher
Elsevier
Publication Date
January 1998
DOI
10.1016/s1074-5521(98)90082-5
ISSN
2451-9456