High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells Academic Article uri icon

  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All


  • Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.


  • DUNCAN, Rory R
  • Don-Wauchope, Andrew
  • TAPECHUM, Sompol
  • SHIPSTON, Michael J
  • CHOW, Robert H
  • ESTIBEIRO, Peter

publication date

  • September 15, 1999