Journal article
High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells
Abstract
Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.
Authors
DUNCAN RR; DON-WAUCHOPE AC; TAPECHUM S; SHIPSTON MJ; CHOW RH; ESTIBEIRO P
Journal
Biochemical Journal, Vol. 342, No. 3, pp. 497–501
Publisher
Portland Press
Publication Date
September 15, 1999
DOI
10.1042/bj3420497
ISSN
0264-6021