High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells Journal Articles

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abstract

Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

authors

DUNCAN, Rory R
Don-Wauchope, Andrew
TAPECHUM, Sompol
SHIPSTON, Michael J
CHOW, Robert H
ESTIBEIRO, Peter

status

published

publication date

September 15, 1999

has subject area

03 Chemical Sciences (FoR)
06 Biological Sciences (FoR)
11 Medical and Health Sciences (FoR)
Adrenal Medulla (MeSH)
Animals (MeSH)
Biochemistry & Molecular Biology (Science Metrix)
Cattle (MeSH)
Chromaffin Cells (MeSH)
Culture Media (MeSH)
Electrophysiology (MeSH)
Exocytosis (MeSH)
Gene Transfer Techniques (MeSH)
Green Fluorescent Proteins (MeSH)
Luminescent Proteins (MeSH)
Mice (MeSH)
Microscopy, Confocal (MeSH)
Polymerase Chain Reaction (MeSH)
Semliki forest virus (MeSH)

published in

Biochemical Journal Journal