Journal article
Solubilization and reconstitution of dopamine D1 receptor from bovine striatal membranes: Effects of agonist and antagonist pretreatment
Abstract
The bovine striatal dopamine D1 receptor was solubilized with a combination of sodium cholate and NaCl in the presence of phospholipids, following treatment of membranes with a dopaminergic agonist (SKF-82526-J) or antagonist (SCH-23390). The solubilized receptors were subsequently reconstituted into lipid vesicles by gel-filtration. A comparison of ligand-binding properties shows that the solubilized and reconstituted receptors bound …
Authors
Srivastava LK; Ross GM; Bajwa SB; Mishra RK
Journal
Neurochemical Research, Vol. 15, No. 6, pp. 647–657
Publisher
Springer Nature
Publication Date
June 1990
DOI
10.1007/bf00973757
ISSN
0364-3190
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepineAnimalsBenzazepinesBinding, CompetitiveCattleCell MembraneCentrifugation, Density GradientCholic AcidCholic AcidsCorpus StriatumDopamine AgentsDopamine AntagonistsFenoldopamGuanylyl ImidodiphosphateLiposomesReceptors, DopamineReceptors, Dopamine D1Solubility