Quantitative and conformational characterization of lysozyme deposited on balafilcon and etafilcon contact lens materials Journal Articles uri icon

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abstract

  • PURPOSE: To determine whether differences in lysozyme deposition and/or activity exist on worn etafilcon and balafilcon contact lenses following care with a polyquaternium-based system (PQ) or a polyhexanide-based system (PHMB). METHODS: Following acid-based deposit extraction, lysozyme concentration was determined via Western blotting and lysozyme activity was determined by a micrococcyl assay. RESULTS: Lysozyme deposition on etafilcon lenses was greater following disinfection with the PHMB-based system (1551 +/- 371 micro g/lens vs 935 +/- 271 micro g/lens; p < 0.001). Deposition on balafilcon lenses was not influenced by the care regimen (10 +/- 3.5 micro g/lens vs 10 +/- 5 micro g/lens; p = 0.89). For both materials, the percentage of denatured lysozyme was greater when they were exposed to the PHMB-based system (28 vs 21%; p = 0.05 (etafilcon) and 57 vs 40%; p = 0.04 (balafilcon)). CONCLUSIONS: The quantity and conformation of lysozyme deposited on hydrogel contact lens materials is significantly influenced by both lens material and care regimen.

authors

  • Senchyna, Michelle
  • Jones, Lyndon
  • Louie, Derek
  • May, Chris
  • Forbes, Iain
  • Glasier, Mary-Ann

publication date

  • January 2004