Bioseparation using supported liquid membrane chromatography

Some of the major problems associated with packed bed chromatography can be solved by using synthetic membranes as chromatographic media. Ion exchange and affinity interactions have mainly been utilised for the separation of proteins using membrane chromatography. In this paper, hydrophobic interaction membrane chromatography of proteins is discussed. A supported liquid membrane was prepared which could bind specific proteins in the presence of high salt concentrations. The separation of CAMPATH-1G monoclonal antibody and BSA using this supported liquid membrane in a chromatographic separation mode is discussed.