Temperature-responsive membrane for hydrophobic interaction based chromatographic separation of proteins in bind-and-elute mode

Butyl-acrylate (BA) containing branched poly-(N-isopropyl acrylamide) or PNIPAM copolymer was grafted on filter paper to prepare composite membrane for carrying out temperature-responsive hydrophobic interaction membrane chromatography (HIMC) of proteins. The copolymer demonstrated two-step phase transition behavior due to the presence of BA and PNIPAM, making the changeover from hydrophilic to hydrophobic state more tunable. The feasibility of …