Electrodeposition of biopolymer films containing haemoglobin

Electrochemical method has been developed for the fabrication of alginic acid–haemoglobin and hyaluronic acid–haemoglobin films. The deposition yield was studied as a function of the haemoglobin concentration in the polymer solutions and deposition time. The results of deposition yield measurements and Fourier transform infrared spectroscopy showed the formation of composite films containing haemoglobin in alginic acid or hyaluronic acid matrix. The haemoglobin content in the composite films was varied by the variation of haemoglobin concentration in the solutions. Electron microscopy studies showed that the film thickness can be varied in the range of 0–50 μm by the variation of deposition time at a constant deposition voltage. The method enabled the formation of uniform films of controlled thickness. The films were deposited as monolayers or multilayers, containing individual alginic acid–haemoglobin and hyaluronic acid–haemoglobin layers. The proposed method can be used for the surface modification of biomedical implants and fabrication of thin films for biosensors.

Electrodeposition of biopolymer films containing haemoglobin Journal Articles