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Negative staining in the detection of viruses in...
Journal article

Negative staining in the detection of viruses in clinical specimens

Abstract

Viruses have unique morphology and are therefore good candidates for negative staining. Negative staining with phosphotungstic acid (PTA) or uranyl acetate has facilitated the detection of many viruses in clinical specimens. Enhancement procedures have included the use of centrifugation and agar diffusion for concentrating virus particles, the use of solid phase capture reagents to trap virus particles and the use of secondary antibodies and …

Authors

Mahony JB; Chernesky MA

Journal

Micron, Vol. 22, No. 4, pp. 449–460

Publisher

Elsevier

Publication Date

1 1991

DOI

10.1016/0739-6260(91)90062-5

ISSN

0968-4328