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Canine traceealis membrane fractionation and...
Journal article

Canine traceealis membrane fractionation and characterization

Abstract

Canine trachealis was homogenized and the various membrane fractions isolated by differential centrifugation and discontinuous sucrose gradient centrifugation. A membrane fraction enriched in the plasma membrane marker enzymes 5′-nucleotidase (5-fold) and K+-activated ouabain sensitive p-nitrophenylphosphatase (3-fold) was obtained. The fraction contained very low levels of the inner mitochondrial marker succinate: cytochrome c oxidoreductase. …

Authors

Grover AK; Kannan MS; Daniel EE

Journal

Cell Calcium, Vol. 1, No. 2, pp. 135–146

Publisher

Elsevier

Publication Date

March 1980

DOI

10.1016/0143-4160(80)90027-5

ISSN

0143-4160