Effect of Matrix Aging on the Behavior of Human Serum Albumin Entrapped in a Tetraethyl Orthosilicate-Derived Glass

The steady-state and time-resolved fluorescence of Trp-214 was used to examine the conformation, dynamics, accessibility, thermal stability, and degree of ligand binding of human serum albumin (HSA) after entrapment of the protein in sol−gel processed glasses. The bioglasses were derived from tetraethyl orthosilicate and were aged in air without washing (dry-aged), in air after a washing step (washed), or in buffer (wet-aged). In all cases, …