Anaplerotic processes in human skeletal muscle during brief dynamic exercise Journal Articles uri icon

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abstract

  • This study examined changes in tricarboxylic acid cycle intermediates (TCAIs) in human skeletal muscle during 5min of dynamic knee extensor exercise (∼80% of maximum workload) and following 2 min of recovery. The sum of the seven measured TCAIs (ΣTCAIs) increased from 1.10 ± 0.08mmol (kg dry weight)−1 at rest to 3.12 ± 0.24, 3.86 ± 0.35 and 4.33 ± 0.30 mmol (kg dry weight)−1after 1, 3 and 5 min of exercise, respectively (P≤ 0.05). The ΣTCAIs after 2 min of recovery (3.74 ± 0.43 mmol (kg dry weight)−1) was not different compared with 5 min of exercise. The rapid increase in ΣTCAIs during exercise was primarily mediated by large changes in succinate, malate and fumarate. These three intermediates accounted for > 90 % of the net increase in ΣTCAIs during the first minute of contraction. Intramuscular alanine increased after 1 min of exercise by an amount similar to the increase in the ΣTCAIs (2.33 mmol (kg dry weight)−1) (P≤ 0.05). Intramuscular pyruvate was also higher (P≤0.05) during exercise, while intramuscular glutamate decreased by ∼50% within 1 min and remained low despite an uptake from the circulation (P≤ 0.05). The calculated net release plus estimated muscle accumulation of ammonia after 1 min of exercise (∼60 μmol (kg wet weight)−1) indicated that only a minor portion of the increase in ΣTCAIs could have been mediated through the purine nucleotide cycle and/or glutamate dehydrogenase reaction. It is concluded that the close temporal relationship between the increase in ΣTCAIs and changes in glutamate, alanine and pyruvate metabolism suggests that the alanine amino‐transferase reaction is the most important anaplerotic process during the initial minutes of contraction in human skeletal muscle.

authors

publication date

  • August 1997