Multiplicity of extracellular -(1,4)-endoglucanases of Bacteroides succinogenes S85

During growth on 0.2% (w/v) microcrystalline cellulose, Bacteroides succinogenes S85 produces endoglucanase activity which can be separated by centrifugation into sedimentable and nonsedimentable fractions. The sedimentable activity, after solubilization with Triton X-100, was resolved into four components by ion-exchange chromatography and these were further fractionated by nondenaturing polyacrylamide gel electrophoresis (PAGE). The …