Flux-dependent transmission of supercoiled plasmid DNA through ultrafiltration membranes

Although previous studies have demonstrated the potential of using membrane-based processes for purification of plasmid DNA, there is considerable discrepancy regarding both the physical mechanisms governing DNA transmission and the effects of membrane pore size and operating conditions on the DNA sieving coefficient. The objective of this work was to obtain quantitative data on the transmission of a 3.0kbp supercoiled plasmid DNA through …