The Kinetics and Affinity of Binding of Glu-Plasminogen Specific to the ϵ-Amino Group ofl-Lysine: Its Potential Application to Modified Biomaterials

By covalently couplingl-lysine to an analytical surface through a dipeptide linkage such that the ϵ-amino group is accessible, we have looked at plasminogen binding in real time. The results have led to a model of plasminogen interactions with ϵ-amino groups. This paper focuses on the desorption kinetics of plasminogen adsorbed to this material. Plasminogen is shown to bind to the dipeptide in at least four different ways to the ϵ-amino groups. …