Optimization of Peak Separation and Broadening in Aqueous Gel Permeation Chromatography (GPC) -Poly (Sodium Styrene Sulfonates)

An experimental study of the gel permeation chromatography of a series of poly (sodium styrene sulfonates) in aqueous media has been undertaken. The objective of this study was to develop a set of operating conditions that would maximize peak separation and minimize peak broadening. Using CPG-10 porous glass packing, it has been found optimal to work at pH near 6 and ionic strength near 0.05. Under those conditions, ion exclusion is eliminated …