Conditions for production of extracellular protein byBacillusbrevis 47

Bacillusbrevis 47 was grown in shake flasks under varying conditions of agitation, culture volume, inoculum size, medium supplementation, and initial pH. It was found to have a high demand for oxygen, and, under appropriate conditions, could produce up to 9 g/l of two extracellular wall proteins in 24 h.