Influence of a paper sizing agent on paper-immobilized biosensing antibodies

The effects of AKD, a paper-sizing agent, on antibody adsorption and inactivation were evaluated. The results showed that paper treated with reasonable content of AKD solution (below 0.092%) actually helped antibody uptake by paper, possibly due to hydrophobic interactions. Further increases in the AKD content, however, suppressed antibody adsorption through the reduction of paper porosity. These results show that antibody-based assays can …