Influence of a paper sizing agent on paper-immobilized biosensing antibodies
Abstract
The effects of AKD, a paper-sizing agent, on antibody adsorption and inactivation were evaluated. The results showed that paper treated with reasonable content of AKD solution (below 0.092%) actually helped antibody uptake by paper, possibly due to hydrophobic interactions. Further increases in the AKD content, however, suppressed antibody adsorption through the reduction of paper porosity. These results show that antibody-based assays can function on sized paper supports typical of packaging materials.