Interaction of NMDA and Dopamine D2L Receptors in Human Neuroblastoma SH‐SY5Y Cells Journal Articles uri icon

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abstract

  • Abstract: To understand the mechanism of interaction of the dopamine D2L receptors with NMDA receptors, we have developed a model by transfecting human neuroblastoma SH‐SY5Y cells with the human dopamine D2L receptor gene. In vitro blockade of NMDA receptors by the specific antagonists MK‐801 and (±)‐3‐(2‐carboxypiperazin‐4‐yl)‐propyl‐1‐phosphonic acid (CPP) on human neuroblastoma SH‐SY5Y cells expressing human dopamine D2L receptors resulted in a significant increase in the density of D2L receptors without a significant change in receptor affinity. Moreover, the dopamine receptor mRNA level increased by ∼50% by the blockade of NMDA with MK‐801. These results suggest a possible interaction of NMDA and dopamine D2L receptors in neuroblastoma SH‐SY5Y cells. This system would serve as an excellent model to study the molecular mechanisms involved in the interaction of these two receptors.

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publication date

  • June 1996